Snippets from My Master’s Journey

September 20, 2025·
Ahmed Massoud
Ahmed Massoud
· 2 min read
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Throughout my master’s journey, I have been fortunate to grow academically and professionally. This page shares selected moments that shaped this experience.

During the pre-master’s year, I completed 10 courses (30 credit hours) and contributed to curriculum preparation and presentations delivered to my colleagues.

By the end of the year, I registered my research point and presented my research proposal on the regulatory roles of miRNAs mediating the analgesic effects of betanin on murine inflammatory pain model.

Research Proposal Seminar

I then began the practical phase of my research, implementing the proposed study design. This phase included animal purchase and acclimatization, induction of the pain model, treatment administration, behavioral assessments, sacrifice and dissection, sample collection, and subsequent molecular, biochemical and ultrastructural analyses, along with in silico simulations.

  • Induction of Pain and Peripheral Inflammation Assessment

Paw Assessment
Paw Thickness Measurement to Assess Peripheral Inflammation
  • Treatment Preparation

Betanin, Purchased from Sigma Aldrich
Treatment Doses Preparation
  • Dissection and Sample Collection

Sample Collection Day
  • Molecular & Biochemical Analysis

  • Molecular

Tissue Lysis Using Qiagen TissueLyser LT
miRNeasy Kit for miRNA Extraction, Purchased from Qiagen
Me Inside the Molecular Biology Lab! 😎
Purity Assessment and Concentration Determination Using NanoDrop2000
Reverse Transcription Using SimpliAmp Thermal Cycler
qRT-PCR Using Qiagen Rotor-gene Q
  • Biochemical

ELISA for Pro and Anti-Inflammatory Markers
While Waiting During the ELISA Incubation Periods! 🫠
Colorimetric Lipid Peroxidation Assessmnet
  • In silico Simulations

Molecular Docking Simulation
Molecular Docking Simulation
  • And finally, data anlysis and representation.

Graphs, Scientific Illustrations, and TE Micrographs

At the end of this journey, and with all praise to Allah, I would like to sincerely acknowledge everyone who supported me throughout—my family, my supervisors, and my colleagues.

Ahmed Massoud
Authors
Teaching Assistant | Researcher | Bio-Scientific Designer
Ahmed Massoud is a molecular biology graduate, ranked first in his major, and currently a full-time Teaching Assistant at the Faculty of Science, Alamein International University. He is also an ASRT MSc Research Scholar under the Scientists for Next Generation (SNG) Program. Alongside his academic background, he has a strong passion for bio-scientific illustration and visual science communication, aiming to simplify complex biological concepts through clear, accurate, and publication-ready figures.